Journal: Frontiers in Pharmacology
Article Title: Targeting a distinct binding pocket in the pregnane X receptor with natural agonist TRLW-2 ameliorates murine ulcerative colitis
doi: 10.3389/fphar.2025.1726597
Figure Lengend Snippet: TRLW-2 promoted the proliferation of MCEC cells in vitro . (A) The apoptosis detection via Annexin V-FITC/PI staining with TRLW-2 and TRLW-5. (B) Representative images of EdU staining (red) showing proliferating cells in the colonic epithelium, the scale bar represents 100 μm. (C) The expression of the proliferation-related markers under the treatment of TRLW-2 and TRLW-5. Data were expressed as the mean ± SD. Data are analyzed by one-way analysis of variance (ANOVA) followed by Dunnett’s test. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.
Article Snippet: Apoptosis was quantified by flow cytometry using an Annexin V-FITC/PI staining kit (A211-01, Vazyme, China).
Techniques: In Vitro, Staining, Expressing